Nanopore device and methods of detecting and classifying charged particles using same

A method of calibrating a nanofluidic device comprising a plurality of nanopore channels, a plurality of gating nanoelectrodes, and a plurality of sensing nanoelectrodes, the method comprising:

• a. applying a selecting voltage across a gating nanoelectrode of the plurality of gating nanoelectrodes to select a nanopore channel corresponding to the gating nanoelectrode;
• b. tuning the nanopore channel by
• applying a first biasing voltage across a sensing electrode of the plurality of sensing nanoelectrodes corresponding to the nanopore channel, and
• receiving a plurality of currents that are distributed over a plurality of probability frequencies from the sensing electrode in response to the applied first biasing voltage;
• c. generating a calibration data set from the pluralities of frequencies and currents;
• d. comparing the calibration data set with a reference data set, wherein the reference data set is a prior set of experimental data or a set of theoretically modelled data;
• e. when the calibration data set differs from the reference data set by more than a predetermined threshold, repeating steps b to d with a second biasing voltage different from the first biasing voltage.