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US 11,686,681 B2Granted
Systems and methods for multicolor imaging
AssigneeSingular Genomics Systems, Inc.
InventorsFedor Trintchouk, Eli N. Glezer
Life of this patent
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Abstract
Disclosed herein, inter alia, are methods and systems of image analysis useful for rapidly identifying and/or quantifying features.
Claims32
An imaging system comprising:
- a sample stage moving at a sample stage speed, wherein the sample stage comprises a sample;
- a first sensor array, and a second sensor array;
- a first optical system configured to direct a first excitation beam and second excitation beam onto a sample;
- a second optical system configured to direct:
- a light emission from said sample to the first sensor array, thereby generating a first charge that transfers across the first sensor array,
- a second light emission from said sample to the second sensor array, thereby generating a second charge that transfers across the second sensor array,
- wherein the transfer of the first charge and the second charge is synchronized with the sample stage speed.
4 more elements
A method of imaging a sample comprising:
- a) directing a first excitation beam and a second excitation beam onto a sample, wherein said sample is on a sample stage moving at a sample stage speed, wherein the sample comprises cell or biological tissue;
- b) directing a first light emission from said sample to impinge upon and generate a first charge that transfers across a first sensor array at a first charge speed, and directing a second light emission from said sample to impinge upon and generate a second charge that transfers across a second sensor array at a second charge speed, wherein at least one of the first charge speed and the second charge speed is synchronized with the sample stage speed;
- and
- c) scanning the sample in a scan dimension and repeating step a) and step b) to form an image of the sample.
1 more element
Dependencies are inferred from the claim text.