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US 11,686,665 B2Granted
Microfluidic system with combined electrical and optical detection for high accuracy particle sorting and methods thereof
AssigneeNodexus Inc.
InventorsHany Nassef, Karthik Balakrishnan, Anand Kesavaraju, Vincent Tuminelli, George Anwar
Life of this patent
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Abstract
Disclosed herein is a system to detect and characterize individual particles and cells using at least either optic or electric detection as the particle or cell flows through a microfluidic channel. The system also provides for sorting particles and cells or isolating individual particles and cells.
Claims21
A particle sorting cartridge, comprising:
- a sorting region comprising an intersection of:
- a first microfluidic channel leading from a sensing region;
- a second microfluidic channel leading from a trigger inlet and substantially perpendicular to the first microfluidic channel at the intersection;
- a default branch channel leading away from the intersection and operably associated with the first microfluidic channel at the intersection;
- and
- a selected branch channel leading away from the intersection at an angle acute to the default branch channel at the intersection and having an inlet with an upstream edge substantially aligned along the first microfluidic channel with an upstream edge of the second microfluidic channel where it meets the first microfluidic channel;
- wherein the sorting region is configured to direct a sample liquid from the sensing region to the default branch channel in absence of a threshold flow of trigger fluid from the trigger inlet through the second microfluidic channel and further configured to direct the sample liquid from the sensing region through the selected branch channel during application of the threshold flow of trigger fluid.
4 more elements
A method for sorting a target particle in a sample fluid stream, the method comprising:
- providing a particle sorting cartridge, comprising:
- a sorting region comprising an intersection of:
- a first microfluidic channel leading from a sensing region;
- a second microfluidic channel leading from a trigger inlet and substantially perpendicular to the first microfluidic channel at the intersection;
- a default branch channel leading away from the intersection and operably associated with the first microfluidic channel at the intersection;
- and
- a selected branch channel leading away from the intersection at an angle acute to the default branch channel at the intersection and having an inlet with an upstream edge substantially aligned along the first microfluidic channel with an upstream edge of the second microfluidic channel where it meets the first microfluidic channel;
- flowing a sample liquid from the sensing region to the default branch channel;
- directing the sample liquid from the sensing region through the selected branch channel by applying a threshold flow of trigger fluid from the trigger inlet through the second microfluidic channel.
6 more elements
Dependencies are inferred from the claim text.