Cytometric device hematology reference composition
AssigneeSlingshot Biosciences, Inc.
InventorsJeffrey Kim, Oliver Liu, Jeremy Agresti, Anh Tuan Nguyen
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Abstract
Hydrogel particles and their use in cytometric applications are described. The hydrogel particles described herein are selectively tunable to have at least one optical property substantially similar to at least one optical property of a target cell. In this regard, the hydrogel particles provided herein, in one aspect, are used as a calibration reagent for the detection of a target cell in a sample.
Claims51
A composition, comprising:
- a suspension fluid;
- a biological cell;
- and
- at least one cell mimic selected from the group consisting of:
- a red blood cell mimic,
- a platelet mimic,
- a lymphocyte mimic,
- a monocyte mimic,
- a reticulocyte mimic,
- a granulocyte mimic,
- a thrombocyte mimic, and
- a nucleated cell mimic,
- wherein the cell mimic is a hydrogel particle comprising a polymerized monomer;
- and
- wherein the cell mimic comprises an optical property that is substantially similar to a corresponding optical property of the cell mimic's natural biological cell counterpart.
A cytometric device hematology reference composition, comprising:
- a suspension fluid;
- a human blood cell biological component;
- and
- at least one synthetic human cell mimic component selected from the group consisting of:
- a human red blood cell mimic,
- a human platelet mimic,
- a human lymphocyte mimic,
- a human monocyte mimic,
- a human reticulocyte mimic,
- a human granulocyte mimic,
- a human thrombocyte mimic, and
- a human nucleated cell mimic,
- wherein the human cell mimic is a hydrogel particle comprising a polymerized monomer;
- and
- wherein the human cell mimic comprises an optical property that is substantially similar to a corresponding optical property of the human cell mimic's natural biological cell counterpart.
A cytometric device hematology reference composition, comprising:
- a suspension fluid;
- and
- at least one synthetic human cell mimic component selected from the group consisting of:
- a human red blood cell mimic,
- a human platelet mimic,
- a human lymphocyte mimic,
- a human monocyte mimic,
- a human reticulocyte mimic,
- a human granulocyte mimic,
- a human thrombocyte mimic, and
- a human nucleated cell mimic,
- wherein the human cell mimic is a hydrogel particle comprising a polymerized monomer;
- and
- wherein the human cell mimic comprises an optical property that is substantially similar to a corresponding optical property of the human cell mimic's natural biological cell counterpart.
A human blood group antigen presenting hydrogel, comprising:
- a) a hydrogel particle functionalized with at least one human blood group antigen.
Dependencies are inferred from the claim text.