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US 11,686,014 B2Granted
Vector library for yeast two hybrid screening and method for identifying deubiquitinating enzyme binding to target protein using same
AssigneeCha University Industry-Academic Cooperation Foundation
InventorsKwang-Hyun Baek, So-Ra Kim, Seul-Ki Kwon, Soo-Yeon Kim, Da-Hye Lee
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Abstract
The present invention provides a vector library for yeast two-hybrid screening of a deubiquitinating enzyme that binds to a target protein and a method for identifying a deubiquitinating enzyme binding to a target protein using the same. Also, the present invention provides a method for screening an agent having anti-cancer activity targeting the deubiquitinating enzyme USP1, USP7, USP12, or USP49 identified by said identifying method.
Claims2
A method for screening an agent having anti-cancer activity, the method comprising:
- (i) treating with candidate materials a cell overexpressing a deubiquitinating enzyme USP1, USP7, USP12, or USP49 and Bax protein, followed by culturing the cell;
- and
- (ii) measuring apoptosis of the cell cultured in Step (i) and selecting a material inducing apoptosis of the cell.
Dependencies are inferred from the claim text.