Method for producing cis-unsaturated fatty acid by recombinant Candida rugosa lipase 1 (rCRL1)

A method for producing cis-unsaturated fatty acid by recombinant Candida rugosa lipase 1 (rCRL1), the method comprising following operations:

• (i) providing an oil-water mixture, wherein the oil-water mixture comprises 1 to 2 parts by weight of oil and 1 part by weight of water;
• (ii) adding 0.02 to 0.25 parts by weight of a recombinant Candida rugosa lipase 1 (rCRL1) into the oil-water mixture;
• (iii) after operation (ii), emulsifying the oil-water mixture without the addition of organic solvents;
• (iv) after operation (iii), hydrolyzing the emulsified oil-water mixture to generate the cis-unsaturated fatty acid;
• (v) after operation (iv), performing an oil-water separation at a temperature of 55° C. to 65° C. and extracting an oil phase layer;
• and
• (vi) after operation (v), performing a cooling and filtering step to obtain the cis-unsaturated fatty acid.