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US 11,685,938 B2Granted
Muconic acid production from genetically engineered microorganisms
AssigneePTT Global Chemical Public Company Limited
InventorsRyan Sillers, Theron Hermann, Michelle Spencer, Russell Udani, R. Rogers Yocum
Life of this patent
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Abstract
The subject of this invention is improvements in the yield and titer of biological production of muconic acid by fermentation. Increased activity of one or more enzymes involved in the muconic acid pathway leads to increased production of muconic acid.
Claims13
A genetically engineered Escherichia coli microorganism that produces muconic acid from a non-aromatic carbon source comprising:
- at least one exogenous gene encoding for 3,4-dihydroxybenzoic acid decarboxylase, a ubiX gene from Escherichia coli encoding for UbiX protein that increases the activity of said 3,4-dihydroxybenzoic acid decarboxylase,
- at least one catAX exogenous gene encoding for catechol 1,2-dioxygenase, and
- at least one exogenous gene encoding for 3-dehydroshikimate dehydratase selected from the group consisting of aroZ, qa4, asbF and quiC.
Dependencies are inferred from the claim text.