Recombinant microorganisms for the enhanced production of mevalonate, isoprene, isoprenoid precursors, isoprenoids, and acetyl-CoA-derived products
AssigneeDanisco US Inc.
InventorsMarguerite A. Cervin, Michael C. Miller, Derek H. Wells, Alan J. Wolfe, Madhukar S. Dasika
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Abstract
The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, isoprenoids, and/or acetyl-CoA-derived products in recombinant microorganisms by engineering the microorganisms to comprise one or more acetylating proteins such that the expression and/or activity of the one or more acetylating proteins is modulated.
Claims35
Recombinant cells capable of producing an isoprenoid precursor, wherein the cells:
- (i) (a) have been modified such that the activity of a YfiQ polypeptide is modulated by decreasing, attenuating, or deleting the expression of a nucleic acid encoding the YfiQ polypeptide as compared to cells that have not been modified for such modulation, and
- (b) comprise a heterologous nucleic acid encoding a CobB polypeptide, wherein the cells have been modified such that the activity of the CobB polypeptide is modulated by increasing the expression of the nucleic acid encoding the CobB polypeptide as compared to cells that have not been modified for such modulation;
- and
- (ii) comprise two or more heterologous nucleic acids encoding two or more polypeptides of a mevalonate (MVA) pathway, wherein culturing of the recombinant cells in a suitable media provides for the production of the isoprenoid precursor;
- and
- (iii) comprise a heterologous nucleic acid encoding a polyprenyl pyrophosphate synthase polypeptide, wherein culturing of the recombinant cells in a suitable media provides for production of the isoprenoid.
The recombinant cells of 1, wherein the isoprenoid precursor production is increased relative to recombinant cells that have not been modified in (i)(a) and (i)(b).
Recombinant cells capable of producing an isoprenoid, wherein the cells:
- (i) (a) have been modified such that the activity of a YfiQ polypeptide is modulated by decreasing, attenuating, or deleting the expression of a nucleic acid encoding the YfiQ polypeptide as compared to cells that have not been modified for such modulation, and
- (b) comprise a heterologous nucleic acid encoding a CobB polypeptide, wherein the cells have been modified such that the activity of the CobB polypeptide is modulated by increasing the expression of the nucleic acid encoding the CobB polypeptide as compared to cells that have not been modified for such modulation;
- (ii) comprise two or more heterologous nucleic acids encoding two or more polypeptides of the MVA pathway;
- and
- (iii) comprise a heterologous nucleic acid encoding a polyprenyl pyrophosphate synthase polypeptide, wherein culturing of the recombinant cells in a suitable media provides for production of the isoprenoid.
Dependencies are inferred from the claim text.