Inducible AAV Rep genes
AssigneeCevec Pharmaceuticals GmbH
InventorsKerstin Hein, Nicole Faust, Silke Wissing
Life of this patent
Estimating the term…
Abstract
The present invention relates to host cells comprising a nucleic acid encoding Adeno-associated virus (AAV) Rep proteins Rep78 and Rep68, wherein the internal AAV promoter p19 has been inactivated by one or more mutations that maintain the functionality of said Rep78 and Rep68 proteins. The present invention further relates to respective nucleic acids and vectors comprising the same, as well as respective methods for the production of AAV.
Claims10
An isolated host cell comprising a nucleic acid encoding Adeno-associated virus (AAV) Rep proteins Rep78 and Rep68 operably linked to an inducible promoter, wherein the internal AAV promoter p19 comprises a substitution at position 818 from a guanine to an adenine wherein the position 818 is numbered according to a complete AAV2 genome of GenBank Accession number AF043303, whereby the internal AAV promoter p19 is inactivated and the functionality of said Rep78 and Rep68 proteins is maintained.
An isolated host cell, comprising a nucleic acid encoding Adeno-associated virus (AAV) Rep proteins Rep78 and Rep68 operably linked to an inducible promoter, wherein the internal AAV promoter p19 comprises a substitution at position 818 from a guanine to an adenine wherein the position 818 is numbered according to a complete AAV2 genome of GenBank Accession number AF043303, whereby the internal AAV promoter p19 is inactivated and the functionality of said Rep78 and said Rep68 proteins is maintained, wherein the nucleic acid also has at least 70% sequence identity to SEQ ID NO:
- 36.
A nucleic acid encoding Adeno-associated virus (AAV) Rep proteins Rep78 and Rep68 operably linked to an inducible promoter, wherein the internal AAV promoter p19 comprises a substitution at position 818 from a guanine to an adenine wherein the position 818 is numbered according to a complete AAV2 genome of GenBank Accession number AF043303, whereby the internal AAV promoter p19 is has been inactivated by one or more mutations that maintain and the functionality of said Rep78 and Rep68 proteins is maintained.
An isolated host cell, comprising a nucleic acid encoding Adeno-associated virus (AAV) Rep proteins Rep78 and Rep68 operably linked to an inducible promoter, wherein the internal AAV promoter p19 comprises a substitution at position 818 from a guanine to an adenine wherein the position 818 is numbered according to a complete AAV2 genome of GenBank Accession number AF043303, whereby the internal AAV promoter p19 is inactivated and the functionality of said Rep78 and said Rep68 proteins is maintained, wherein the nucleic acid also has at least 70% sequence identity to SEQ ID NO:
- 45.
Dependencies are inferred from the claim text.