Systems, methods, and compositions for targeted nucleic acid editing
AssigneesThe Broad Institute, Inc., Massachusetts Institute of Technology, President and Fellows of Harvard College
InventorsFeng Zhang, Jonathan Gootenberg, David Benjamin Turitz Cox, Omar Abudayyeh, Soumya Kannan
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Abstract
The disclosure provides for systems, methods, and compositions for targeting and editing nucleic acids. In particular, the invention provides non-naturally occurring or engineered RNA-targeting systems comprising a RNA-targeting Cas13 protein, at least one guide molecule, and at least one adenosine deaminase protein or catalytic domain thereof.
Claims6
An engineered, non-naturally occurring system comprising a catalytically inactive Cas13b effector protein (dCas13b) or a nucleotide sequence encoding the catalytically inactive Cas13b effector protein, wherein the truncated form of the Cas13b effector protein has been truncated at:
- N-terminal Δ1-20, N-terminal Δ1-40, N-terminal Δ1-60, N-terminal Δ1-80, N-terminal Δ1-100, N-terminal Δ1-120, N-terminal Δ1-140, N-terminal Δ1-160, N-terminal Δ1-180, N-terminal Δ1-200, N-terminal Δ1-220, N-terminal Δ1-240, N-terminal Δ1-260, or N-terminal Δ1-300, wherein amino acid positions of the truncations correspond to amino acid positions of Prevotella sp. P5-125 Cas13b protein as set forth in SEQ ID NO:
- 82.
An engineered, non-naturally occurring system comprising a catalytically inactive Cas13b effector protein (dCas13b) or a nucleotide sequence encoding the catalytically inactive Cas13b effector protein, wherein the truncated form of the Cas13b effector protein has been truncated at:
- C-terminal Δ1070-1090, C-terminal Δ1050-1090, C-terminal Δ1030-1090, C-terminal Δ1010-1090, C-terminal Δ990-1090, C-terminal Δ970-1090, C-terminal Δ950-1090, C-terminal Δ930-1090, C-terminal Δ910-1090, C-terminal Δ890-1090, C-terminal Δ870-1090, C-terminal Δ850-1090, C-terminal Δ830-1090, C-terminal M90-1090, C-terminal Δ984-1090, C-terminal Δ1026-1090, C-terminal Δ1053-1090, C-terminal Δ934-1090, C-terminal Δ884-1090, C-terminal Δ834-1090, C-terminal M84-1090, or C-terminal M34-1090 wherein amino acid positions of the truncations correspond to amino acid positions of Prevotella sp. P5-125 Cas13b protein as set forth in SEQ ID NO:
- 82.
Dependencies are inferred from the claim text.