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US 11,685,915 B2Granted
Method of collecting nucleic acid
AssigneeToray Industries, Inc.
InventorsShota Sekiguchi, Taiga Arai, Masateru Itou
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Abstract
A method utilizes an aluminum oxide support with a water-soluble neutral polymer adsorbed on a surface of the aluminum oxide support and is aimed to collect nucleic acids from a body fluid sample. The method includes a step of adsorbing nucleic acids on the support in the presence of a chaotropic agent and a step of adding a solution containing an anionic surfactant to the nucleic acid-adsorbed support.
Claims10
A method of collecting a nucleic acid(s) from a body fluid sample, the method comprising:
- step a) mixing a chaotropic agent and an aluminum oxide support with a water-soluble neutral polymer adsorbed on a surface of the aluminum oxide support with a solution containing a nucleic acid(s), and adsorbing the nucleic acid(s) to the support;
- step b) separating the support on which the nucleic acid(s) is/are adsorbed from the solution mixed at step a);
- step c) mixing the support separated at step b) with a solution containing 0.1 wt % to 2 wt % of an anionic surfactant;
- step d) separating the support on which the nucleic acid(s) is/are adsorbed from the solution mixed at step c);
- and
- step e) adding an eluent to the support separated at step d) and collecting the nucleic acid(s).
3 more elements
Dependencies are inferred from the claim text.