Stabilised dry protein deamidase composition
AssigneeAmano Enzyme Inc.
InventorTakakiyo Obara
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Abstract
A technique of using a compositional innovation of a dry enzyme composition to improve the stability of a protein deamidase composition enables improvement of the stability of a dry protein deamidase composition by making both a protein deamidase and magnesium chloride coexist in a dry enzyme composition. The technique involves making adjustments so that the pH is at least 2 and less than 5 when the composition is dissolved in water at a concentration of 1 w/v %.
Claims4
A dry stabilized composition comprising a dry protein deamidase and magnesium chloride,
- wherein the dry stabilized composition has a deamidation activity after storage for one month at 40° C. of at least 60% as compared to a deamidation activity of the dry stabilized composition stored for one month at −20° C.,
- wherein an amino acid sequence of the protein deamidase has identity of 80% or more with the amino acid sequence represented by SEQ ID NO:
- 1 or 2, and
- wherein a content of the magnesium chloride in the dry stabilized composition is from 2.5% to 6.5% by weight.
A stability-improving method for improving stability of a protein deamidase in a dry enzyme composition including the protein deamidase, wherein the protein deamidase coexists with the magnesium chloride in the dry enzyme composition, or
- a pH of the dry enzyme composition upon dissolution in water at 1 w/v % is adjusted to 2 or higher and lower than 5.
A dry stabilized composition comprising a dry protein deamidase having a pH of 2 or higher and 4 or lower upon dissolution in water at 1 w/v %,
- wherein the dry stabilized composition has a deamidation activity after storage for one month at 40° C. of at least 60% as compared to a deamidation activity of the dry stabilized composition stored for one month at −20° C.,
- wherein an amino acid sequence of the protein deamidase has an identity of 80% or more with an amino acid sequence represented by SEQ ID NO:
- 1 or 2, and
- wherein a stabilizing agent other than an agent for adjusting the pH is not added.
Dependencies are inferred from the claim text.