T4 DNA ligase variants with increased resistance to salt
AssigneeAbClonal Science, Inc.
InventorsAlicia DiCicco, Zhenyu Zhu, Aine Quimby
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Abstract
A number of T4 DNA ligase mutants exhibiting enhanced ligation activity in the presence of high salt concentrations compared to the wild-type ligase were engineered, characterized, and selected via gel electrophoresis of ligation products from a standard ligation assay. Ligase catalyzes the formation of phosphodiester bonds between the 5′ and 3′ ends of complementary cohesive ends or blunt ends of duplex DNA, a process that is vital to numerous molecular biology processes including cloning and sequencing.
Claims9
A mutant T4 DNA ligase having 95% sequence identity to SEQ ID NO:4 and comprising the amino acid substitution E88K.
A mutant T4 DNA ligase having 99% sequence identity to SEQ ID NO:4 and comprising the amino acid substitution E88K.
Dependencies are inferred from the claim text.