Enzymatic production of alpha-1,3-glucan
AssigneeNutrition & Biosciences USA 4, Inc.
InventorsKevin D. Nagy, Susan Marie Hennessey, Yefim Brun, Michael Reichman
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Abstract
A method for producing insoluble alpha-1,3-glucan is disclosed. Embodiments of the method comprise providing (i) oligosaccharides that comprise alpha-1,3 and alpha-1,6 glycosidic linkages, or (ii) oligosaccharides derived from a glucosyltransferase reaction; and contacting at least water, sucrose, a glucosyltransferase enzyme, and the oligosaccharides provided in the first step. Glucosyltransferase reaction compositions embodying such a method, and insoluble products thereof, are also disclosed. Yield and other product benefits can be realized when practicing the disclosed subject matter.
Claims19
A method for producing insoluble alpha-1,3-glucan comprising:
- (a) providing filtrate or supernatant of a glucosyltransferase reaction that produces insoluble alpha-1,3-glucan having at least 50% alpha-1,3 glycosidic linkages;
- and
- (b) contacting at least sucrose, said filtrate or supernatant, and a glucosyltransferase enzyme that produces insoluble alpha-1,3-glucan having at least 50% alpha-1,3 glycosidic linkages, thereby producing said insoluble alpha-1,3-glucan;
- wherein
- the glucosyltransferase of the glucosyltransferase reaction of step (a) comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:2, 4, 6, 7, 8, or 9,
- and the glucosyltransferase of step (b) comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:2, 4, 6, 7, 8, or 9.
Dependencies are inferred from the claim text.