Nanofiber-based long-term primary hepatocyte three-dimensional culture system and culturing method
AssigneeNanofaentech Co., Ltd.
InventorsJong-Young Kwak, Min Gyu Song, So Hee Kim, Min Ho Choi
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Abstract
Disclosed is a nanofiber-based long-term primary hepatocyte culture system and a culture method, wherein the primary hepatocyte culture system has an advantage that it can culture cells in three-dimensions in vitro to maintain the original physiological activity of low proliferative primary hepatocytes for a long time by co-culturing indirectly by separating primary hepatocytes and hepatic non-parenchymal cells with a support consisting of nanofibers therebetween without direct co-culture.
Claims11
A three-dimensional co-culture system of primary hepatocytes and liver non-parenchymal cells comprising:
- a transwell comprising a membrane of polyvinyl alcohol (PVA) nanofibers, wherein the primary hepatocytes are attached to the membrane of PVA nanofibers;
- and
- a culture plate comprising a support of polycaprolactone (PCL) nanofibers, wherein the liver non-parenchymal cells are attached to the support of PCL nanofibers,
- wherein the transwell and the culture plate are stacked adjacently, and
- wherein the transwell and the culture plate prevent a direct co-culture between the primary hepatocytes and the liver non-parenchymal cells and induce an indirect co- culture.
A three-dimensional co-culture method for primary hepatocytes and liver non-parenchymal cells comprising:
- culturing primary hepatocytes on a membrane of PVA nanofibers in a transwell, wherein the primary hepatocytes are attached to the membrane of the PVA nanofibers;
- culturing liver non-parenchymal cells on a support of PCL nanofibers attached to a culture plate;
- stacking the transwell comprising the primary hepatocytes and the culture plate comprising the liver non-parenchymal cells;
- and
- co-culturing the primary hepatocytes and the liver non-parenchymal cells,
- wherein the transwell and the culture plate prevent a direct co-culture between the primary hepatocytes and the liver non-parenchymal cells and induce an indirect co- culture.
Dependencies are inferred from the claim text.