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US 11,685,899 B2Granted
Cell culture method for mesenchymal stem cells
AssigneeMesoblast International Sarl
InventorsPaul Simmons, Colby Suire
Life of this patent
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Abstract
The present disclosure relates to methods, cell culture medium and compositions that promote cell proliferation during fetal bovine serum free cell culture.
Claims11
An in vitro method of cell culture, the method comprising:
- culturing a population of mesenchymal lineage precursor cells or stem cells in a fetal bovine serum free cell culture medium comprising platelet derived growth factor (PDGF), epidermal growth factor (EGF) and fibroblast growth factor 2 (FGF2), wherein the level of PDGF is 10 ng/ml or less, the level of EGF is 5 ng/mi or less, and the level of FGF2 is 1 ng/ml or less, wherein the method increases mesenchymal lineage precursor cell or stem cell proliferation.
Dependencies are inferred from the claim text.