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US 11,685,835 B2Granted
Ultra bright dimeric or polymeric dyes
AssigneeSony Corporation
InventorTracy Matray
Life of this patent
Estimating the term…
Abstract
Compounds useful as fluorescent or colored dyes are disclosed. The compounds have the following structure (I): or a stereoisomer, tautomer or salt thereof, wherein R1, R2, R3, R4, R5, L, L1, L2, L3, L4, M, M′, m1, m2, n, x, y and z are as defined herein. Methods associated with preparation and use of such compounds are also provided.
Claims17
A compound having the following structure (I):
- or a salt or tautomer thereof, wherein:
- M is, at each occurrence, independently a monovalent moiety comprising two or more carbon-carbon double bonds and at least one degree of conjugation;
- M′ is, at each occurrence, independently a divalent moiety having one of the following structures:
- L is, at each occurrence, independently an alkylene or alkenylene linker, wherein the alkylene or alkenylene linker is unsubstituted;
- L2 and L3 are, at each occurrence, independently an optional alkylene, alkenylene, alkynylene, heteroalkylene, heteroalkenylene, heteroalkynylene or heteroatomic linker;
- L1 is at each occurrence, independently either:
- i) an optional alkylene, alkenylene, alkynylene, heteroalkylene, heteroalkenylene, heteroalkynylene or heteroatomic linker;
- or ii) a linker comprising a functional group capable of formation by reaction of two complementary reactive groups;
- L4 is, at each occurrence, independently an alkylene, alkenylene, alkynylene, heteroalkylene, heteroalkenylene, carbocyclic or heterocyclic linker;
- R1 is, at each occurrence, independently H, alkyl or alkoxy;
- R2 and R3 are each independently H, OH, SH, alkyl, alkoxy, alkylether, heteroalkyl, —OP(═Ra)(Rb)Rc, Q or L′;
- R4 is, at each occurrence, independently OH, SH, O−, S−, ORd or SRd;
- R5 is, at each occurrence, independently oxo, thioxo or absent;
- Ra is O or S;
- Rb is OH, SH, O−, S−, ORd or SRd;
- Rc is OH, SH, O−, S−, ORd, OL′, SRd, alkyl, alkoxy, heteroalkyl, heteroalkoxy, alkylether, alkoxyalkylether, phosphate, thiophosphate, phosphoalkyl, thiophosphoalkyl, phosphoalkylether or thiophosphoalkylether;
- Rd is a counter ion;
- Q is, at each occurrence, independently a moiety comprising a reactive group, or protected analogue thereof, capable of forming a covalent bond with an analyte molecule, a targeting moiety, a solid support or a complementary reactive group Q′;
- L′ is, at each occurrence, independently a linker comprising a covalent bond to Q, a linker comprising a covalent bond to a targeting moiety, a linker comprising a covalent bond to an analyte molecule, a linker comprising a covalent bond to a solid support, a linker comprising a covalent bond to a solid support residue, a linker comprising a covalent bond to a nucleoside or a linker comprising a covalent bond to a further compound of structure (I);
- m1 and m2 are, at each occurrence, independently an integer of zero or greater;
- n, x and y are each independently an integer of zero or greater for each integral value of z;
- and
- z is an integer of 1 or greater,
- provided that x is 1 or greater for at least one integral value of z, and at least one of n or y is 1 or greater for at least one integral value of z.
21 more elements
A compound having the following structure:
- wherein y is an integer greater than 1.
Dependencies are inferred from the claim text.