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US 11,685,772 B2Granted
Mammalian cell culture
AssigneeAmgen Inc.
InventorsBrian D. Follstad, Rebecca E. McCoy, Arvia E. Morris
Life of this patent
Estimating the term…
Abstract
The invention provides a method for culturing mammalian cells. The method provides greater control over cell growth to achieve high product titer cell cultures.
Claims30
A method of producing a monoclonal antibody, comprising:
- establishing a Chinese Hamster Ovary (CHO) cell culture in a serum-free culture medium in a bioreactor by inoculating the bioreactor with at least 0.5×106 to 3.0×106 cells/mL, wherein said CHO cell expresses said monoclonal antibody,
- limiting the viable cell density (VCD) of said culture at 1×108 cells/mL by perfusion with a serum-free perfusion medium having an L-asparagine concentration of 5 mM or less,
- maintaining the CHO cells by perfusion with a serum-free perfusion medium having an L-asparagine concentration of 5 mM or less, such that the VCD of the culture is maintained at 1×108 cells/mL or less.
A method of producing denosumab, comprising:
- establishing a Chinese Hamster Ovary (CHO) cell culture in a serum-free culture medium in a bioreactor by inoculating the bioreactor with at least 0.5×106 to 3.0×106 cells/mL, wherein said CHO cell expresses denosumab,
- limiting the viable cell density (VCD) of said culture at 1×108 cells/mL by perfusion with a serum-free perfusion medium having an L-asparagine concentration of 5 mM or less,
- maintaining the CHO cells by perfusion with a serum-free perfusion medium having an L-asparagine concentration of 5 mM or less, such that the VCD of the culture is maintained at 1×108 cells/mL or less.
A method of producing panitumumab, comprising:
- establishing a Chinese Hamster Ovary (CHO) cell culture in a serum-free culture medium in a bioreactor by inoculating the bioreactor with at least 0.5×106 to 3.0×106 cells/mL, wherein said CHO cell expresses panitumumab,
- limiting the viable cell density (VCD) of said culture at 1×108 cells/mL by perfusion with a serum-free perfusion medium having an L-asparagine concentration of 5 mM or less,
- maintaining the CHO cells by perfusion with a serum-free perfusion medium having an L-asparagine concentration of 5 mM or less, such that the VCD of the culture is maintained at 1×108 cells/mL or less.
Dependencies are inferred from the claim text.