Bone protein extraction without demineralization
AssigneeRensselaer Polytechnic Institute
InventorsTimothy Paul Cleland, Deepak Vashishth
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Abstract
Embodiments of the invention relate generally to protein extraction and, more generally, to bone protein extraction methods that do not require demineralization. In one embodiment, the invention provides a method comprising: mixing a bone sample and a quantity of an extraction buffer comprising: ammonium phosphate dibasic; or ammonium phosphate dibasic and ammonium bicarbonate; or ammonium phosphate dibasic, ammonium bicarbonate, and guanidine HCl; or sodium phosphate dibasic and sodium bicarbonate; or sodium phosphate dibasic, sodium bicarbonate, and guanidine HCl; or potassium phosphate dibasic and potassium bicarbonate; or potassium phosphate dibasic, potassium bicarbonate, and guanidine HCl; and incubating the bone sample/extraction buffer mixture.
Claims4
A bone protein extraction buffer effective to solubilize matrix proteins from a sample of solid bone, comprising:
- ammonium phosphate dibasic, ammonium bicarbonate, guanidine HCl, and at least one protease inhibitor, wherein the at least one protease inhibitor is selected from the group consisting of:
- sodium fluoride, sodium orthovanadate, sodium pyrophosphate, beta-glycerophosphate, and mixtures thereof,
- wherein the bone protein extraction buffer does not include a demineralization agent, and the extraction buffer includes ammonium phosphate dibasic at a concentration of 400 mM.
Dependencies are inferred from the claim text.