Device and method for intracellular delivery of biomolecular cargo via acoustic wave exposure

A microfluidic device for the intracellular delivery of biomolecular cargo into cells or enveloped viruses comprising:

• a piezoelectric substrate having a microfluidic channel disposed on a surface thereof and fluidically coupled to a solution containing cells or enveloped viruses;
• a pump configured to pump the solution containing the cells or enveloped viruses into an inlet of the microfluidic channel;
• a first pair of interdigitated electrodes (IDTs) disposed on opposing sides of the microfluidic channel at a first location along the microfluidic channel;
• a second pair of interdigitated electrodes (IDTs) disposed on opposing sides of the microfluidic channel at a second location along the microfluidic channel, the second location being located downstream of the first location;
• a signal generator configured to apply an AC voltage to the first pair of IDTs and when actuated generates bulk acoustic waves (BAWs) that focus the cells or enveloped viruses at a lateral position of the microfluidic channel and configured to apply an AC voltage to the second pair of IDTs and when actuated causes permeabilization of the cells or enveloped viruses which effectuates transfection of the cells or enveloped viruses.

A microfluidic device for the intracellular delivery of biomolecular cargo into cells or enveloped viruses comprising:

• a piezoelectric substrate having a plurality of microfluidic channels disposed on a surface thereof, the plurality of microfluidic channels fluidically coupled to a solution containing cells or enveloped viruses;
• a pump configured to pump the solution containing the cells or enveloped viruses into the plurality of microfluidic channels;
• a first plurality of respective pairs of interdigitated electrodes (IDTs) disposed on opposing sides each of the plurality of microfluidic channels at a first location along the plurality of microfluidic channels;
• a second plurality of respective pairs of interdigitated electrodes (IDTs) disposed on opposing sides of each of the plurality of microfluidic channels at a second location along the plurality of microfluidic channels, the second location being located downstream of the first location;
• a signal generator configured to apply an AC voltage to the first plurality of respective pairs of interdigitated electrodes (IDTs) and the second plurality of respective pairs of interdigitated electrodes (IDTs), wherein actuation of the first plurality of respective pairs of IDTs focuses the cells or enveloped viruses at a lateral location within the plurality of microfluidic channels and wherein actuation of the second plurality of respective pairs of IDTs causes permeabilization of the cells or enveloped viruses which effectuates transfection of the cells or enveloped viruses.