Tight connection device for the aseptic transfer of a biopharmaceutical product between a chamber and a container

A tight connection device to ensure the aseptic transfer of a biopharmaceutical product between a chamber equipped with a removable door and a container equipped with a removable cover, comprising:

• a stationary temporary clamping means configured to hold the container against the chamber such that the removable cover of said container is hermetically pressed against the removable door of said chamber;
• a stationary unlocking means for switching the container from an initial locking position wherein the removable cover hermetically seals the container to an intermediate unlocking position wherein the removable cover is disengaged from the container and hermetically held against the removable door of the chamber so as to ensure an aseptic communication between said container and said chamber;
• a stationary locking means for switching the container from the intermediate unlocking position to an end locking position wherein said removable cover again hermetically seals the container;
• an annular functional crown rotatable about a geometric axis of rotation so as to actuate the stationary unlocking means and the stationary locking means of the container, said stationary unlocking means and said stationary locking means being mechanically linked to the annular functional crown and arranged such that the rotation of said annular functional crown about the geometric axis of rotation successively drives the actuation of the stationary unlocking means ensuring the switching into the intermediate unlocking position of the container, then the actuation of the stationary locking means ensuring the switching into the end locking position of the container, and
• wherein the device further comprises stationary immobilizing/release means fixedly coupled to the chamber and configured to prevent rotation of the annular functional crown with the stationary immobilizing/release means in an immobilizing position, and to allow rotation of the annular functional crown with the stationary immobilizing/release means in a release position, wherein the stationary immobilizing/release means remains fixedly coupled to the chamber in the immobilizing position and in the release position.