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US 11,684,676 B2Granted
Site-specific antibody-drug conjugates by ADP-ribosyl cyclases
AssigneeUniversity of Southern California
InventorsYong Zhang, Zhefu Dai, Xiao-Nan Zhang
Life of this patent
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Abstract
Antibody-drug conjugates, compositions thereof, and methods use. The antibody-drug conjugates include a fusion protein comprising an antibody covalently linked to an ADP-ribosyl cyclase protein via a peptide linker moiety at one or more of a C-terminus or N-terminus of a heavy or light chain of the antibody, a NAD or NMN analogue, and a payload such that the NAD or NMN analogue is conjugated to both the payload and the ADP-ribosyl cyclase protein.
Claims25
An antibody-drug conjugate comprising:
- a fusion protein comprising an antibody covalently linked to an ADP-ribosyl cyclase protein via a peptide linker moiety;
- a 2′-modified araNAD+ analogue or a 2′-modified araNMN analogue;
- and
- a payload wherein the 2′-modified araNAD+ analogue or the 2′-modified araNMN analogue is conjugated to the payload to form a functionalized payload, and the functionalized payload is conjugated to the ADP-ribosyl cyclase protein.
1 more element
A method of preparing an antibody-drug conjugate comprising:
- providing an antibody-peptide linker-ADP-ribosyl cyclase fusion protein;
- combining a 2′-modified araNAD+ analogue or a 2′-modified araNMN analogue and a payload moiety to form a 2′-modified araNAD+ analogue-payload or a 2′-modified araNMN analogue-payload;
- and
- conjugating the antibody-peptide linker-ADP-ribosyl cyclase fusion protein to the 2′-modified araNAD+ analogue-payload or the 2′-modified araNMN analogue-payload to form the antibody-drug conjugate.
1 more element
Dependencies are inferred from the claim text.