Methods and compositions employing immunogenic fusion proteins

A method for producing a fusion protein comprising expressing in a cell an isolated polynucleotide that encodes the fusion protein, said fusion protein comprising

• a) a first polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:
• 3 or SEQ ID NO:
• 4, wherein said first polypeptide forms a loop conformation and is immunogenic,
• b) a second polypeptide having at least one T cell epitope (TCE), and comprising a pneumolysoid having an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
• 7 and is immunogenic, and
• c) a third polypeptide comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
• 3 or SEQ ID NO:
• 4, wherein said third polypeptide forms a loop conformation and is immunogenic;
• wherein said fusion protein comprises said first, said second, and said third polypeptide in the following order:
• said first polypeptide operably linked to said second polypeptide operably linked to said third polypeptide and wherein said second polypeptide is heterologous to said first and said third polypeptides.