Treatment of a bacterial vaginal infection

#This application is a § 371 national stage of PCT International Application No. PCT/EP2018/058508, filed Apr. 3, 2018, claiming priority of European Patent Application No. EP 17461524.5, filed Apr. 14, 2017, the contents of each of which are hereby incorporated by reference into this application.

#The present invention relates to the field of medicine. In particular, the present invention relates to use of furazidin to treat of bacterial infections of the vagina.

#Bacterial vaginosis (bacterial vaginal infection), and bacterial vaginitis is most often caused by a bacterial infection or has a mixed etiology (bacterial+fungal and/or protozoal infection). The infection results from an abnormal growth of bacteria of the Lactobacillus spp. strain on the surface of the vaginal epithelium and the qualitative change in a composition of the bacteria of the vagina. Bacteria from the Lactobacillus spp. strain, due to a strong adhesion to the vaginal epithelium and their ability to produce the lactic acid protect the environment of the vagina against bacterial and fungal infections. In the majority of cases, bacterial vaginosis and mixed infections of the vagina are caused by the anaerobic bacteria Gardnerella vaginalis and Atopobium vaginae. Interestingly, in more than 90% of cases, a biofilm formed on the epithelial cells of the vagina of women with bacterial vaginoses, was composed of both Gardnerella vaginalis, and Atopobium vaginae.

#Analysis of the antimicrobial activity of furazidin, nitrofurantoin, nifuratel, clindamycin metronidazole, and nystatin was performed to determine the minimum concentration which inhibits the growth of microorganisms—minimal inhibitory concentration values (MIC) using the method described below. Strains used in the study were Gardnerella vaginalis ATCC 14018, Atopobium vaginae ATCC BAA 55, Candida albicans ATCC 90028, and Candida glabrata ATCC 2001.

#A stock solution for all of compounds was 10000 μg/ml, from which 15 serial dilutions were prepared (500 μg/mL, 250 μg/mL, 125 μg/mL, 62.5 μg/mL, 31.25 μg/mL, 15.6 μg/mL, 7.8 μg/mL, and 3.9 μg/mL, 1.95 μg/mL, 0.98 μg/mL, 0.49 μg/mL, 0.24 μg/mL, 0.12 μg/mL, 0.06 μg/mL, and 0.03 μg/mL).

#Macro Dilution Method for Bacteria in a Solid Medium