Multi-vesicular liposomes for targeted delivery of drugs and biologics for tissue engineering
AssigneeKarma Biotechnologies
InventorsAndrew Brovko Gray, Donald Dewane Johnson, Alan Michael Johnson
Life of this patent
Estimating the term…
Abstract
A multivesicular liposome composition includes a plurality of multivesicular liposomes. Each multivesicular liposome includes a carrier liposome particle including carrier phospholipid bilayer and having an average diameter less than 1 micron; and at least one sub-chamber liposome nanoparticle including a sub-chamber liposome nanoparticle bilayer and having an average diameter less than about 50 nm. The carrier liposome particle encapsulates the at least one sub-chamber liposome nanoparticle. Characteristically, tail groups of the carrier liposome phospholipids are larger than tail groups phospholipids of the at least one sub-chamber liposome nanoparticle.
Claims26
A multivesicular liposome composition including a plurality of multivesicular liposomes, each multivesicular liposome comprising:
- a carrier liposome particle including a carrier phospholipid bilayer comprising carrier phospholipids and having an average diameter less than 1 micron;
- and
- at least one sub-chamber liposome nanoparticle including a sub-chamber phospholipid bilayer comprising sub-chamber phospholipids and having an average diameter less than about 50 nm, the carrier liposome particle encapsulating the at least one sub-chamber liposome nanoparticle, wherein tail groups of the carrier phospholipids are larger on average than the tail groups of the sub-chamber phospholipids, wherein the carrier liposome particles includes crosslinks between phospholipids in the same layer of the carrier phospholipid bilayer and crosslinks between different layers of the carrier phospholipid bilayer and wherein at least one sub-chamber liposome nanoparticle includes crosslinks between phospholipids in the same layer of the sub-chamber phospholipid bilayer and crosslinks between different layers of the sub-chamber phospholipid bilayer.
A method of forming a multivesicular liposome composition including a plurality of multivesicular liposomes, each multivesicular liposome comprising:
- a carrier liposome particle including a carrier phospholipid bilayer comprising carrier phospholipids and having an average diameter less than 1 micron;
- and
- at least one sub-chamber liposome nanoparticle including a sub-chamber phospholipid bilayer comprising sub-chamber phospholipids and having an average diameter less than about 50 nm, the carrier liposome particle encapsulating the at least one sub-chamber liposome nanoparticle, wherein tail groups of the carrier phospholipids are larger on average than the tail groups of the sub-chamber phospholipids, the method comprising:
- a) forming a plurality of sub-chamber liposome nanoparticles, the plurality of sub-chamber liposome nanoparticles having an average nanoparticle diameter less than about 50 nm, the plurality of sub-chamber liposome nanoparticles being formed from a sub-chamber liposome-forming composition that includes a first payload;
- and
- b) forming a plurality of carrier liposome particles having an average nanoparticle diameter less than about 1 micron, the plurality of carrier liposome particles being formed from a carrier liposome-forming composition that includes the plurality of sub-chamber liposome nanoparticles such that sub-chamber liposome nanoparticles are encapsulated by carrier liposome particles.
A carrier liposome composition including a plurality of carrier liposomes particles, each carrier liposomes particle comprising:
- a carrier phospholipid bilayer comprising carrier phospholipids and having an average diameter less than 1 micron.
Dependencies are inferred from the claim text.