Method, process, composition and kit for measuring concentration of molecules dissolved in the continuous phase of a colloid

A method for measuring the concentration of molecules, characterized in that said method measures the concentration of molecules dissolved in the continuous phase of a colloid and comprises the steps of:

• a) obtaining a test sample by mixing a number of molecules with a volume of colloid;
• b) obtaining a control sample by mixing a number of molecules with a volume of a composition comprising a particle-free solution extracted from a fraction of the continuous phase of same colloid used in step a), so that a value of the concentration of molecules in the mixture is equal to the value of the concentration of molecules in the test sample obtained in step a);
• c) submitting the test and the control samples obtained in steps a) and b) to a process in order to concentrate the particles of the test sample obtained in step a);
• d) recovering a particle-free volume from the test sample obtained in step c);
• e) recovering a volume from the control sample obtained in step c);
• f) measuring in the particle-free volume recovered from the test sample in step d) the value of a parameter proportional to the number of molecules added in step a);
• g) measuring in the volume recovered from the control sample in step e) the value of the same parameter measured in step f);
• and
• h) measuring the concentration of molecules in the continuous phase of the colloid using the equation:
• i
• )
• ⁢
• [
• FC
• ]
• =
• [
• ]
• 0
• ⁢
• @
• mp
• @
• mc
• wherein [FC] corresponds to the concentration of molecules dissolved in the continuous phase of a colloid, [ ]0 corresponds to the value of the concentration of molecules used in the steps a) and b), @mp corresponds to an empirical value of the parameter proportional to the number of molecules contained in the particle-free volume recovered from the test sample obtained in step f), and @mc corresponds to an empirical value of the same parameter, but measured in the volume recovered from the control sample obtained in step g).