Methods for terpenoid production
AssigneesAgency for Science, Technology and Research, Justus-Liebig-Universitaet Giessen
InventorsCongqiang Zhang, Xixian Chen, Ruehl Martin, Heng-Phon Too
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Abstract
A bacterial strain comprising one or more vectors encoding a) one or more enzymes to produce one or more terpene precursors; and b) a fungal terpene synthase (FTPS). The present invention also relates to a method of producing a terpenoid comprising a) culturing the bacterial strain described herein in an expression medium; and b) isolating the terpenoid from said expression medium.
Claims20
A bacterial strain comprising one or more vectors encoding
- a) one or more enzymes to produce one or more terpene precursors;
- and
- b) a fungal terpene synthase (FTPS), wherein the FTPS is an Agrocybe aegerita FTPS comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 1, 20, 26, 27, 28, 29, 30, 31, 32, 33 and 34.
A bacterial strain comprising one or more vectors encoding
- a) one or more enzymes to produce one or more terpene precursors;
- and
- b) a fungal terpene synthase (FTPS) comprising an amino acid sequence having at least 90% identity to an amino acid sequence selected from the group consisting of SEQ ID NO:
- 1, 20, 26, 27, 28, 29, 30, 31, 32, 33 and 34.
A genetically engineered 1-deoxyxylulose-5-phosphate synthase (DXS), wherein the genetically engineered DXS comprises an amino acid sequence comprising mutations E210D, Q459L and L415T in the amino acid sequence set forth in SEQ ID NO:
- 6, optionally wherein the genetically engineered DXS further comprises a mutation H105T.
Dependencies are inferred from the claim text.