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US 11,680,081 B2Granted
Method for preparation of a separation matrix
AssigneeCytiva BioProcess R&D AB
InventorsGustav Rodrigo, Mats Ander, Ronnie Palmgren, Tomas Bjorkman
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Abstract
Methods that include providing and reacting a solid support and an alkali-stable ligand derived from an immunoglobulin-binding bacterial protein to form a separation matrix having covalently coupled alkali-stable ligands; and washing with a wash solution comprising at least 10 mM of an alkali metal hydroxide.
Claims48
A method for preparation of a separation matrix, comprising the steps of:
- a) providing a solid support and an alkali-stable ligand derived from a bacterial immunoglobulin-binding protein;
- b) reacting said alkali-stable ligand with said solid support to form a separation matrix having covalently coupled alkali-stable ligands;
- and
- c) washing said separation matrix having covalently coupled alkali-stable ligands with a wash solution comprising at least 10 mM of an alkali metal hydroxide;
- wherein the separation matrix is substantially free from non-covalently coupled ligands derived from said bacterial immunoglobulin-binding protein;
- and
- wherein said alkali-stable ligand derived from a bacterial immunoglobulin-binding protein is capable of withstanding incubation with 0.1 M NaOH for 100×10 min at 22+/−2° C. with less than a 45% reduction in binding capacity towards IgG.
4 more elements
Dependencies are inferred from the claim text.