Compounds, reagents, and uses thereof
AssigneeMetabolon, Inc.
InventorQibo Zhang
Life of this patent
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Abstract
The present invention provides a compound of formula (I), formula (II), formula (III), (IV) or a salt thereof, compositions and methods of making the compound, methods and reagents for measuring the compound, and kits using the same. The use of a compound of formula (I), formula (II), formula (III), or formula (IV) for assessing or monitoring kidney function in a subject, determining predisposition to developing reduced kidney function, classifying a subject according to level of kidney function, and diagnosing or monitoring chronic kidney disease is also described.
Claims20
A compound represented by the following formula:
- or a salt thereof, wherein *indicates 13C;
- and the compound is at least 60% pure.
A method for preparing a compound represented by the following formula:
- or a salt thereof, comprising the steps of:
- a) reacting a compound represented by the following formula:
- or a salt thereof, with a Wittig reagent R′3P═CH2SMe, wherein R′ is Ph or an electron withdrawing group, to form a compound of formula (Va) or a salt thereof:
- b) deprotecting the compound of (Va) or a salt thereof to form a compound of formula (I) or a salt thereof.
A method of preparing a compound represented by the following formula:
- or a salt thereof, wherein
- (i) the method comprises the steps of:
- a) reacting a compound of formula (VII):
- or a salt thereof, with a Wittig reagent R′3P═CH2SMe, to form a compound of formula (VIIa),
- or a salt thereof, wherein R′ is Ph or an electron-withdrawing group, and R1 and R2 are each independently a silyl group;
- b) purifying the compound of formula (VIIa) or a salt thereof, to yield a compound of formula (VIIb) or a salt thereof:
- c) deprotecting the compound of formula (VIIb) or a salt thereof to form the compound of formula (III) or a salt thereof;
- or
- (ii) the method comprises the steps of:
- a) reacting a compound of formula (VII):
- or a salt thereof, with a Wittig reagent R′3P═CH2SMe, to form a compound of formula (VIIa) or a salt thereof,
- wherein R′ is Ph or an electon-withdrawing group, and R1 and R2 are each independently a silyl group;
- b) deprotecting the compound of formula (VIIa) or a salt thereof to form a compound of formula (VIIc) or a salt thereof:
- c) purifying the compound of formula (VIIc) or a salt thereof to yield the compound of formula (III) or a salt thereof.
A method of preparing a compound represented by the following formula:
- or a salt thereof, wherein
- (i) the method comprises the steps of:
- a) reacting a compound of formula (IX):
- with methanethiol to form a compound of formula (VIIIa) or a salt thereof:
- b) purifying the compound of formula (VIIIa) or a salt thereof to yield a compound of formula (VIIIb) or a salt thereof:
- c) deprotecting the compound of formula (VIIIb) or a salt thereof to form the compound of formula (III) or a salt thereof, or
- (ii) the method comprises the steps of:
- a) reacting a compound of formula (IX):
- with methanethiol to form a compound of formula (VIIIa) or a salt thereof:
- b) deprotecting the compound of formula (VIIIa) or a salt thereof to form a compound of formula (VIIIb) or a salt thereof:
- c) purifying the compound of formula (VIIIb) or a salt thereof to yield the compound of formula (III) or a salt thereof,
- wherein:
- R1 and R2 are each independently a silyl group and R3 is a t-butyl group or a silyl group.
Dependencies are inferred from the claim text.