Differentiation of natural vitamin E from synthetic vitamin E and quantification of tocopherols by supercritical fluid chromatography
AssigneeWaters Technologies Corporation
InventorsJinchuan Yang, Paul Rainville
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Abstract
The present disclosure provides methods for the separation and quantitation of enantiomers of vitamin E using supercritical fluid chromatography (SFC) or carbon dioxide-based chromatography on chiral columns. The disclosed methods may be used to quantitatively determine the concentration of RRR-α-tocopherol in foods, food ingredients, dietary supplements, vitamin premixes, nutritional formulas, and medicines. Further provided is a method of differentiating the source of α-tocopherol as natural or synthetic.
Claims15
A method for the chiral separation of stereoisomers of α-tocopherol or α-tocopheryl acetate in a sample, the method comprising:
- a. providing a sample comprising at least one stereoisomer of α-tocopherol or α-tocopheryl acetate;
- b. contacting said sample with a column chromatography device comprising a first column having an interior portion at least partially filled with an immobilized stationary phase, wherein the immobilized stationary phase comprises amylose tris(3,5-dimethylphenylcarbamate), cellulose tris(3,5-dimethylphenylcarbamate), or cellulose tris(3-chloro-4-methylphenylcarbamate);
- c. flowing a mobile phase through the immobilized stationary phase for a period of time, the mobile phase comprising carbon dioxide and a co-solvent which is a combination of acetonitrile and water;
- and
- d. eluting the at least one stereoisomer of α-tocopherol or α-tocopheryl acetate from the immobilized stationary phase in the mobile phase;
- and
- e. detecting the presence of the at least one stereoisomer of α-tocopherol or α-tocopheryl acetate using a detector.
Dependencies are inferred from the claim text.