Agricultural compositions comprising remodeled nitrogen fixing microbes
AssigneePivot Bio, Inc.
InventorsMark Reisinger, Ernest Sanders, Richard Broglie, Karsten Temme
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Abstract
The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmospheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer. The remodeled microbes taught herein are able to be combined with leading agricultural chemistry and elite germplasm. Furthermore, the disclosure provides seed treatments comprising remodeled microbes.
Claims23
A composition, comprising:
- a) a plurality of non-intergeneric remodeled bacteria;
- and
- b) at least one plant hormone selected from the group consisting of auxins, cytokinins, gibberellins, abscisic acid, ethylene, brassinosteroids, jasmonic acid, and strigolactones;
- wherein the non-intergeneric remodeled bacteria produce fixed N of at least about 1×10−17 mmol N per bacterial cell per hour;
- wherein the non-intergeneric remodeled bacteria have an average colonization ability per unit of plant root tissue of at least about 1.0×104 bacterial cells per gram of fresh weight of plant root tissue;
- wherein a member of the plurality of non-intergeneric remodeled bacteria comprises at least one genetic variation introduced into a gene selected from the group consisting of:
- nifA, nifL, ntrB, ntrC, polynucleotide encoding glutamine synthetase, glnA, glnB, glnK, drat, amtB, polynucleotide encoding glutaminase, glnD, glnE, nifJ nifH, nifD, nifK, nifY, nifE, nifN, nifU, nifS, nifV, nifW, nifZ, nifM, nifF, nifB, nifQ, a gene associated with biosynthesis of a nitrogenase enzyme, and combinations thereof.
Dependencies are inferred from the claim text.