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US 11,678,667 B2Granted
Agricultural compositions comprising remodeled nitrogen fixing microbes
AssigneePivot Bio, Inc.
InventorsMark Reisinger, Ernest Sanders, Richard Broglie, Karsten Temme
Life of this patent
Estimating the term…
Abstract
The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmostpheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer. The remodeled microbes taught herein are able to be combined with leading agricultural chemistry and elite germplasm. Furthermore, the disclosure provides seed treatments comprising remodeled microbes.
Claims126
A seed treatment composition, comprising:
- a) a plurality of non-intergeneric remodeled bacteria;
- and
- b) at least one compound of Formula (1) or a salt, solvate, or isomer thereof,
- wherein Formula (1) comprises:
- each E is independently O, S, or —NR7;
- each G is independently C or N;
- R1, R4, R5, and R6 are each independently H, amino, halo, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, —OR8, —C(O)R8,
- or a lone electron pair, wherein
- indicates a single bond;
- R2 and R3 are each independently H, amino, halo, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl, substituted or unsubstituted cycloalkyl, or substituted or unsubstituted heterocycloalkyl, or a lone electron pair;
- or R2 and R3 together form a bond, or form a substituted or unsubstituted aryl;
- and
- R7 and R8 are each independently H, amino, halo, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl, substituted or unsubstituted cycloalkyl, or substituted or unsubstituted heterocycloalkyl.
10 more elements
A seed treatment composition, comprising:
- a) a plurality of non-intergeneric remodeled bacteria, which produce fixed N of at least about 1×10−17 mmol N per bacterial cell per hour and have an average colonization ability per unit of plant root tissue of at least about 1.0×104 bacterial cells per gram of fresh weight of plant root tissue;
- and wherein a member of the plurality of non-intergeneric remodeled bacteria comprises at least one genetic variation introduced into a gene selected from the group consisting of:
- nifA, nifL, ntrB, ntrC, polynucleotide encoding glutamine synthetase, glnA, glnB, glnK, drat, amtB, polynucleotide encoding glutaminase, glnD, glnE, nifJ, nifH, nifD, nifK, nifY, nifE, nifty, nifU, nifS, nifV, nifW, nifZ, nifM, nifF, nifB, nifQ, a gene associated with biosynthesis of a nitrogenase enzyme, and combinations thereof;
- and
- b) at least one agricultural compound selected from the group consisting of:
- i) a strigolactone compound or mimic thereof;
- and
- ii) a compound of Formula (1) or a salt, solvate, or isomer thereof,
- wherein Formula (1) comprises:
- each E is independently O, S, or —NR7;
- each G is independently C or N;
- R1, R4, R5, and R6 are each independently H, amino, halo, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, —OR8, —C(O)R8,
- or a lone electron pair, wherein
- indicates a single bond;
- R2 and R3 are each independently H, amino, halo, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl, substituted or unsubstituted cycloalkyl, or substituted or unsubstituted heterocycloalkyl, or a lone electron pair;
- or R2 and R3 together form a bond, or form a substituted or unsubstituted aryl;
- and
- R7 and R8 are each independently H, amino, halo, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl, substituted or unsubstituted cycloalkyl, or substituted or unsubstituted heterocycloalkyl.
15 more elements
A composition, comprising:
- a) a corn seed, wherein the corn seed is a genetically modified corn seed, wherein said genetically modified corn seed comprises a herbicide tolerance trait and/or an insect resistance trait;
- b) a plurality of non-intergeneric remodeled bacteria;
- and
- c) at least one agricultural compound selected from the group consisting of:
- i) a strigolactone compound or mimic thereof;
- and
- ii) a compound of Formula (1) or a salt, solvate, or isomer thereof,
- wherein Formula (1) comprises:
- each E is independently O, S, or —NR7;
- each G is independently C or N;
- R1, R4, R5, and R6 are each independently H, amino, halo, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, —OR8, —C(O)R8,
- or a lone electron pair, wherein
- indicates a single bond;
- R2 and R3 are each independently H, amino, halo, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl, substituted or unsubstituted cycloalkyl, or substituted or unsubstituted heterocycloalkyl, or a lone electron pair;
- or R2 and R3 together form a bond, or form a substituted or unsubstituted aryl;
- and
- R7 and R8 are each independently H, amino, halo, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl, substituted or unsubstituted cycloalkyl, or substituted or unsubstituted heterocycloalkyl.
14 more elements
A composition, comprising:
- a) a plurality of non-intergeneric remodeled bacteria,
- wherein a member of the plurality of non-intergeneric remodeled bacteria comprises at least one genetic variation introduced into a gene selected from the group consisting of:
- nifA, nifL, ntrB, ntrC, polynucleotide encoding glutamine synthetase, glnA, glnB, glnK, drat, amtB, polynucleotide encoding glutaminase, glnD, glnE, nifJ, nifH, nifD, nifK, nifY, nifE, nifty, nifU, nifS, nifV, nifW, nifZ, nifM, nifF, nifB, nifQ, a gene associated with biosynthesis of a nitrogenase enzyme, and combinations thereof;
- and
- b) at least one agricultural compound selected from the group consisting of:
- i) a strigolactone compound or mimic thereof;
- and
- ii) a compound of Formula (1) or a salt, solvate, or isomer thereof,
- wherein Formula (1) comprises:
- each E is independently O, S, or —NR7;
- each G is independently C or N;
- R1, R4, R5, and R6 are each independently H, amino, halo, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, —OR8, —C(O)R8,
- or a lone electron pair, wherein
- indicates a single bond;
- R2 and R3 are each independently H, amino, halo, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl, substituted or unsubstituted cycloalkyl, or substituted or unsubstituted heterocycloalkyl, or a lone electron pair;
- or R2 and R3 together form a bond, or form a substituted or unsubstituted aryl;
- and
- R7 and R8 are each independently H, amino, halo, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl, substituted or unsubstituted cycloalkyl, or substituted or unsubstituted heterocycloalkyl.
15 more elements
A seed treatment composition, comprising:
- a) a plurality of non-intergeneric remodeled Kosakonia;
- and
- b) at least one agricultural compound selected from the group consisting of:
- i) a strigolactone compound or mimic thereof;
- and
- ii) compound of Formula (1) or a salt, solvate, or isomer thereof,
- wherein Formula (1) comprises:
- each E is independently O, S, or —NR7;
- each G is independently C or N;
- R1, R4, R5, and R6 are each independently H, amino, halo, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, —OR8, —C(O)R8,
- or a lone electron pair, wherein
- indicates a single bond;
- R2 and R3 are each independently H, amino, halo, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl, substituted or unsubstituted cycloalkyl, or substituted or unsubstituted heterocycloalkyl, or a lone electron pair;
- or R2 and R3 together form a bond, or form a substituted or unsubstituted aryl;
- and
- R7 and R8 are each independently H, amino, halo, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl, substituted or unsubstituted cycloalkyl, or substituted or unsubstituted heterocycloalkyl.
13 more elements
A composition, comprising:
- a) a corn seed;
- b) a plurality of non-intergeneric remodeled bacteria;
- wherein a member of the plurality of non-intergeneric remodeled bacteria comprises at least one genetic variation introduced into a gene selected from the group consisting of:
- nifA, nifL, ntrB, ntrC, polynucleotide encoding glutamine synthetase, glnA, glnB, glnK, drat, amtB, polynucleotide encoding glutaminase, glnD, glnE, nifJ, nifH, nifD, nifK, nifY, nifE, nifty, nifU, nifS, nifV, nifW, nifZ, nifM, nifF, nifB, nifQ, a gene associated with biosynthesis of a nitrogenase enzyme, and combinations thereof;
- and
- c) at least one agricultural compound selected from the group consisting of:
- i) a strigolactone compound or mimic thereof;
- and
- ii) a compound of Formula (1) or a salt, solvate, or isomer thereof,
- wherein Formula (1) comprises:
- each E is independently O, S, or —NR7;
- each G is independently C or N;
- R1, R4, R5, and R6 are each independently H, amino, halo, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, —OR8, —C(O)R8,
- or a lone electron pair, wherein
- indicates a single bond;
- R2 and R3 are each independently H, amino, halo, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl, substituted or unsubstituted cycloalkyl, or substituted or unsubstituted heterocycloalkyl, or a lone electron pair;
- or R2 and R3 together form a bond, or form a substituted or unsubstituted aryl;
- and
- R7 and R8 are each independently H, amino, halo, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl, substituted or unsubstituted cycloalkyl, or substituted or unsubstituted heterocycloalkyl.
16 more elements
Dependencies are inferred from the claim text.