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US 11,674,161 B2Granted
Modified monooxygenases for the manufacture of hydroxylated hydrocarbons
AssigneeCovestro Intellectual Property GmbH & Co. KG
InventorsRuijing Guo, Jen-Chieh Lin, Sha Tao, Ying Qian, Chenggang Qiu, Kequan Chen, Kang Li
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Abstract
The present invention relates to novel monooxygenases which are useful in the hydroxylation of aromatic hydrocarbons. They are particularly useful for the production of 1-naththol and 7-hydroxycoumarin from naphthol and 7-Ethoxycoumarin, respectively.
Claims11
A modified P450 monooxygenase having an amino acid sequence with at least 90% sequence identity with SEQ ID NO:
- 1, wherein the asparagine at position 199 of the amino acid sequence of SEQ ID NO. 1 is substituted by an amino acid selected from the group consisting of glutamine, isoleucine, leucine, phenylalanine, histidine, methionine, arginine, serine, threonine, tyrosine, tryptophan, alanine, valine, and lysine to provide a functional mutation, wherein said functional mutation leads to an improved reactivity on hydroxylation of aromatic hydrocarbons as compared to a non-modified P450 monooxygenase.
Dependencies are inferred from the claim text.