Pharmaceutical chimeric receptor composition and method thereof

An expression vector of an artificial chimeric antigen receptor (CAR), comprising:

• a CD 19 antigen-binding fragment sequence comprising a nucleic acid sequence encoding a heavy chain variable domain, a light chain variable domain and a linker,
• wherein the nucleic acid sequence of the heavy chain variable domain is SEQ ID NO:
• 2 and the nucleic acid sequence of the light chain variable domain is SEQ ID NO:
• 1,
• or the nucleic acid sequence of the heavy chain variable domain is SEQ ID NO:
• 4 and the nucleic acid sequence of the light chain variable domain is SEQ ID NO:
• 3,
• or the nucleic acid sequence of the heavy chain variable domain is SEQ ID NO:
• 6 and the nucleic acid sequence of the light chain variable domain is SEQ ID NO:
• 5;
• a transmembrane domain sequence comprising a nucleic acid sequence encoding a transmembrane sequence of CD28, IgG1, CD4, CD8a or any combination thereof;
• and a signaling domain sequence comprising a nucleic acid sequence encoding at least one immunoreceptor tyrosine-based activation motif (ITAM) sequence, at least one co-stimulatory molecule (CM) sequence, or combination thereof.