Back to results
US 11,667,968 B2Granted
Fragmentation of DNA
AssigneeNew England Biolabs, Inc.
InventorsLynne Apone, Brittany S. Sexton, Margaret Heider, Louise J S Williams, Eileen T. Dimalanta
Life of this patent
Estimating the term…
Abstract
Provided herein is a polymerase-free enzyme mix (FRAG) for fragmenting double-stranded DNA. In some embodiments the enzyme mix may comprise a double-stranded DNA nickase and at least one of a DNA ligase capable of sealing a nick within a DNA, and a single-strand specific DNA nuclease. Methods for fragmenting double-stranded DNA are also provided.
Claims14
A polymerase-free reaction mix for fragmenting double-stranded DNA, comprising:
- (a) a nickase that nicks double-stranded DNA;
- (b) a single-strand DNA nuclease;
- and
- (c) a DNA ligase capable of sealing a nick within a DNA;
- and
- (d) double-stranded DNA to be fragmented,
- wherein and the reaction mix does not contain a DNA polymerase.
4 more elements
A method comprising:
- (a) combining, in a first container, a sample comprising double-stranded DNA with a DNA polymerase-free enzyme mix that comprises;
- (i) a nickase that nicks double-stranded DNA at random sites;
- (ii) a single-strand DNA nuclease;
- and
- (iii) a DNA ligase capable of sealing a nick within a DNA;
- to produce a reaction mix;
- and
- (b) incubating the reaction mix to provide fragments of the double-stranded DNA.
5 more elements
Dependencies are inferred from the claim text.