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US 11,667,945 B2Granted
Detection method and detection probe for colibactin and colibactin-producing bacteria
AssigneeShizuoka Prefecture Public University Corporation
InventorsKenji Watanabe, Yuta Tsunematsu, Michio Sato
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Abstract
The present invention provides a method and probe for determining colibactin and a colibactin-producing bacterium. According to the present invention, there is provided a fluorescent probe for detecting myristoyl asparagine using, for example, a tissue sample and a fecal sample and detecting enzyme activity of ClbP.
Claims6
A compound or a fluorescent probe selected from the group consisting of:
- (A) a compound or a fluorescent probe obtained by connecting an amino group of a coumarin fluorescent dye having the amino group at position 7 of a coumarin skeleton and a carboxyl group of myristoyl asparagine via an amide bond;
- (B) a compound or a fluorescent probe obtained by connecting an amino group of a naphthalimide fluorescent dye having the amino group at position 4 of a naphthalimide skeleton and a carboxyl group of myristoyl asparagine via an amide bond;
- (C) a compound or a fluorescent probe obtained by connecting an amino group of naphthalene, pyrene or anthracene having the amino group at position 2 thereof and a carboxyl group of myristoyl asparagine via an amide bond;
- (D) a compound or a fluorescent probe obtained by connecting one or both of amino groups of a rhodamine fluorescent dye having the amino group at position 3 and/or 9 of a xanthene skeleton and carboxyl group(s) of myristoyl asparagine via an amide bond;
- (E) a compound or a fluorescent probe obtained by connecting one or both of amino groups of Bodipy fluorescent dye having the amino group at position 1 and/or 7 of Bodipy mother nucleus and the carboxyl group(s) of myristoyl asparagine via an amide bond;
- (F) a compound or a fluorescent probe obtained by connecting said carboxyl group and said amino group in any one of (A) to (E) via alanine (—NH—CH(CH3)-CO—) (each forms an amide bond with alanine);
- (G) a compound or a fluorescent probe obtained by connecting a carboxyl group of myristoyl asparagine and a hydroxyl group of oFlu, which is a fluorescent group that exhibits fluorescence when the hydroxyl group is released, via a self-cleavable linker, wherein the selfcleavable linker is connected to the carboxyl group of myristoyl asparagine via an amide bond;
- and the hydroxyl group of oFlu is liberated when the amide bond is decomposed by hydrolysis;
- and
- (H) a compound or fluorescent probe having a fluorescent dye connected to a carboxyl group of myristoyl asparagine via an amide bond, wherein fluorescence is emitted after the amide bond is cleaved in a ClbP dependent manner.
7 more elements
Dependencies are inferred from the claim text.