Production and use of phosphoethanolamine cellulose and derivatives
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsLynette Cegelski, Wiriya Thongsomboon
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Abstract
Phosphoethanolamine cellulose and methods of making and using it are disclosed. In particular, the invention relates to a method of producing a phosphoethanolamine cellulose biosynthetically using a BcsG phosphoethanolamine transferase for cellulose modification. Recombinant constructs encoding BcsG are described, including constructs encoding BcsG by itself or in combination with BcsE and BcsF, which increase the extent of cellulose modification and the amount of modified cellulose produced. Production of phosphoethanolamine cellulose in cell culture and derivatization of phosphoethanolamine cellulose are also described.
Claims19
A method of producing phosphoethanolamine cellulose, the method comprising:
- a) culturing an Escherichia or a Salmonella host cell comprising a recombinant polynucleotide encoding a BcsG phosphoethanolamine transferase comprising the amino acid sequence of SEO ID NO:12 or SEO ID NO:13 operably linked to a promoter under conditions suitable for expression of the BcsG phosphoethanolamine transferase, wherein the phosphoethanolamine cellulose is produced;
- and
- b) isolating the phosphoethanolamine cellulose.
Dependencies are inferred from the claim text.