Method of selectively differentiating undifferentiated macrophage into M1 macrophage

A method for selective differentiation of undifferentiated macrophages into M1 macrophages, the method comprising incubating the undifferentiated macrophages in an incubator under a pressurized environment;

• wherein the pressurized environment is created by a transwell or a pressing plate which vertically descends from an upper portion of the incubator to the inside of the incubator to mechanically press the top surface of said undifferentiated macrophages at a pressure corresponding to atmospheric pressure of 1.5-5;
• wherein the transwell comprises:
• a hollow chamber;
• and a membrane which is provided in an empty space of the chamber and is composed of a porous film;
• wherein a hydrogel is attached to a bottom surface of the membrane or the pressing plate;
• wherein the hydrogel is formed by hydration of alginate and a concentration of the alginate is 2%, and wherein the incubating the undifferentiated macrophages in the incubator under a pressurized environment is for at least 48 hours.