Recombinant yeast cell
AssigneeDsm IP Assets B.V.
InventorsIoannis Papapetridis, Jacobus Thomas Pronk
Life of this patent
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Abstract
The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein one or more genes of the non-oxidative branch of the pentose phosphate pathway are overexpressed and/or wherein said yeast cell comprises a deletion or disruption of a glycerol-3-phosphate dehydrogenase (GPD) gene.
Claims16
A recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (Rubisco) having EC number 4.1.1.39 and further comprising one or more phosphoribulokinase (PRK) having EC number 2.7.1.19, wherein:
- one or more genes of the non-oxidative branch of the pentose phosphate pathway, and encoding an enzyme selected from the group consisting of transaldolase having EC number 2.2.1.2, transketolase having EC number 2.2.1.1, ribose-5-phosphate isomerase having EC number 5.3.1.6 and D-ribulose-5-phosphate 3-epimerase having EC number 5.1.3.1, are overexpressed;
- the PRK is under control of a promoter which has a PRK expression ratioanaerobic/aerobic of 2 or more;
- and
- the Rubisco and the one or more genes of the non-oxidative branch of the pentose phosphate pathway are under control of one or more constitutive promoters, and
- the recombinant yeast cell further comprising a deletion or disruption of a glycerol-3-phosphate dehydrogenase (GPD) gene.
Dependencies are inferred from the claim text.