Back to results
US 11,667,677 B2Granted
Inducible, tunable, and multiplex human gene regulation using CRISPR-Cpf1
AssigneeThe General Hospital Corporation
InventorsY. Esther Tak, Benjamin Kleinstiver, J. Keith Joung
Life of this patent
Estimating the term…
Abstract
Drug-inducible, tunable, and multiplexable Clustered Regularly Interspaced Short Palindromic Repeats from Prevotella and Francisella 1 (Cpf1)-based activators, and methods of use thereof.
Claims14
A composition comprising:
- (a) a first fusion protein comprising a catalytically inactive Lachnospiraceae bacterium ND2006 Cpf1 (dLbCpf1) fused to more than one first dimerization domains, and (b) a second fusion protein comprising at least one activation domain fused to a second dimerization domain that dimerizes with at least one of the first dimerization domains in the first fusion protein in the presence of a dimerizing agent, wherein the first and second dimerization domains are rapamycin analog A/C heterodimerizer DmrA or DmrC.
Dependencies are inferred from the claim text.