US 11,666,882 B2 · Bidirectional flow reaction system for solid phase synthesis

Abstract

Flow control mechanisms control the direction and flow rate of synthesis reagent through one or more synthesis reaction vessels for automated solid phase synthesis. Selectable, known, and reproducible positive or negative pressure differentials (−5 to +10 psi) accomplish controlled, bidirectional (forward and reverse) flow of synthesis reagents through synthesis media contained within the reaction vessels. Venturi-based vacuum apparatus, valves, electronic pressure regulators and compound digital pressure gauge, can be added to automated solid phase synthesis instruments to provide, control, and monitor known, selectable, reproducible negative and positive pressures to one or both valve sealable and un-sealable ends (inlets and outlets) of the reaction vessel as needed to generate and reverse said pressure differentials between the opposite ends of said synthesis reaction vessels, yielding controlled forward and backward flows of synthesis reagents through the synthesis media.

Claims22

Dependent claims

A bidirectional flow reaction system for solid phase synthesis, comprising one or more generators of gas pressure differential across opposite ends of at least one reaction vessel containing porous, solid synthesis media and a liquid reagent having been introduced thereto, the liquid reagent having reactants that react with active sites of the synthesis media while in contact with the active sites, alternating net gas pressure differential relative to a zero-flow equilibrium pressure differential yielding a reproducible bidirectional flow of liquid reagent through the synthesis media that keeps the liquid reagent within the reaction vessel and maintains contact between reactants and active sites for a specified time duration,

wherein the at least one reaction vessel has at least one valve at one end thereof which is alternately connectable to one of the generators of net gas pressure differential and to a vent open to atmospheric pressure to yield the alternating net gas pressure differential.

A method of controlling bidirectional flow of reagent through a reaction vessel, comprising:

introducing a liquid reagent into at least one reaction vessel containing a solid synthesis media, the liquid reagent having reactants that react with active sites of the synthesis media while in contact with the active sites;
and
generating a reversing gas pressure differential across opposite ends of the at least one reaction vessel, the reversing gas pressure differential yielding an alternating forward and backward flow of reproducible volumes of liquid reagent through the synthesis media that keeps the liquid reagent within the reaction vessel and maintains contact between reactants and active sites for a specified time duration,
wherein the reversible gas pressure differential across opposite ends of the at least one reaction vessel is created by alternately applying a net gas pressure and a controlled venting to atmospheric pressure at one end of the reaction vessel, the opposite end of the reaction vessel being sealed, the applying of the net gas pressure producing a reagent flow through the synthesis media from a higher pressure end to a lower pressure end of the reaction vessel until a pressure equilibrium is reached with the sealed end, the controlled venting to atmospheric pressure then producing a reverse reagent flow through the synthesis media until a pressure equilibrium is again reached.

1 more element

Dependencies are inferred from the claim text.

Bidirectional flow reaction system for solid phase synthesis

Life of this patent

Bidirectional flow reaction system for solid phase synthesis

Life of this patent

Abstract

Claims22