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US 11,666,662 B2Granted
Silica-based biomolecule carrier, pharmaceutical composition comprising the same, preparation method and use thereof
AssigneeNational Taiwan University
InventorsChung-Yuan Mou, Yi-Ping Chen, Si-Han Wu
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Abstract
Silica-based biomolecule carriers, compositions comprising the same and preparation methods and uses thereof for delivering biomolecules into a cell are provided. The silica-based biomolecule carrier comprises a porous core; a first bioactive moiety; a second bioactive moiety functionally associated with the first bioactive moiety; and linkers for respectively conjugating the first bioactive moiety and the second bioactive moiety to the porous core.
Claims12
A silica-based biomolecule carrier, comprising:
- a silica-based porous core;
- a first bioactive moiety;
- a second bioactive moiety functionally associated with the first bioactive moiety;
- and
- linkers which separately conjugate each of the first bioactive moiety and the second bioactive moiety to the silica-based porous core;
- wherein the first bioactive moiety is an anti-NF-κB (nuclear factor-kappa B) p65 antibody and the second bioactive moiety is a TAT transducing peptide comprising an amino acid sequence selected from:
- SEQ ID NO. 1 and SEQ ID NO. 2, such that the silica-based biomolecule carrier moves near nuclear membrane and blocks nuclear translocation, and wherein the silica-based porous core has a particle size of at least 40 nm.
4 more elements
A method of preparing a composition comprising a silica-based biomolecule carrier, comprising:
- providing a silica-based carrier having a porous core;
- forming linkers on the silica-based porous core;
- conjugating first biomolecules to the silica-based porous core via at least a part of the linkers;
- and
- conjugating second biomolecules functionally associated with the first biomolecules to the silica-based porous core via at least a part of the linkers;
- and
- forming the silica-based biomolecule carrier, the silica-based biomolecule carrier comprising a first bioactive moiety, which is formed by linking the first biomolecules to the silica-based porous core, and a second bioactive moiety, which is formed by linking the second biomolecules to the silica-based porous core;
- wherein the first bioactive moiety is an anti-NF-κB (nuclear factor-kappa B) p65 antibody and the second bioactive moiety is a TAT transducing peptide comprising an amino acid sequence selected from:
- SEQ ID NO. 1 and SEQ ID NO. 2, such that the silica-based carrier moves near nuclear membrane and blocks nuclear translocation, and wherein the silica-based porous core has a particle size of at least 40 nm.
6 more elements
Dependencies are inferred from the claim text.