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US 11,662,343 B2Granted
Cellular populations and uses thereof
AssigneeThe Council of the Queensland Institute of Medical Research
InventorsGeoffrey R. Hill, Ping Zhang
Life of this patent
Estimating the term…
Abstract
Disclosed are methods of identifying immunosuppressive TR1 regulatory T cells, including in methods of diagnosing the presence of immune tolerance, methods of producing immunosuppressive regulatory T cells, and methods of eliciting immune tolerance in a subject. These methods include screening T cells to detect Eomes+IL-10+ T cells or expressing recombinant Eomes in T cell populations to generate immunosuppressive regulatory T cells.
Claims13
A method of producing a population of immunosuppressive regulatory T cells, the method comprising:
- isolating a heterogenous population of cells comprising regulatory T cells;
- enriching for IL-10+ CD4+ T cells that are optionally positive or high for at least one of CD122, α4β7, LAG-3, Ly6C and TIGIT, and/or optionally negative or low for one or more of CD25, CD69 and FoxP3;
- and introducing into the IL-10+ CD4+ T cells a construct comprising an Eomes coding sequence in operable connection with a regulatory sequence that is operable in the IL-10+ CD4+ T cells, to thereby produce a population of immunosuppressive regulatory T cells comprising Eomes+IL-10+ CD4+ T cells.
Dependencies are inferred from the claim text.