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US 11,661,617 B2Granted
Processes for the production of tryptamines
AssigneeCompass Pathfinder Limited
InventorRyan Protzko
Life of this patent
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Abstract
Disclosed herein are prokaryotic and eukaryotic microbes, including E. coli and S. cerevisiae, genetically altered to biosynthesize tryptamine and tryptamine derivatives. The microbes of the disclosure may be engineered to contain plasmids and stable gene integrations containing sufficient genetic information for conversion of an anthranilate or an indole to a tryptamine. The fermentative production of substituted tryptamines in a whole-cell biocatalyst may be useful for cost effective production of these compounds for therapeutic use.
Claims10
A composition comprising:
- (a) an engineered microbial cell containing therein one or more heterologous nucleic acid sequences encoding one or more of:
- (i) a tryptamine 4-hydroxylase comprising an amino acid sequence having at least 80% sequence identity to any one of SEQ ID NOS:
- 32-35;
- (ii) a tryptamine 5-hydroxylase comprising an amino acid sequence having at least 80% sequence identity to SEQ ID NO:
- 47;
- (iii) a kinase comprising an amino acid sequence having at least 80% sequence identity to any one of SEQ ID NOS:
- 41-44;
- (iv) a P450 reductase comprising an amino acid sequence having at least 80% sequence identity to any one of SEQ ID NOS:
- 36-40;
- and
- (v) a transferase comprising an amino acid sequence having at least 80% sequence identity to any one of SEQ ID NOS:
- 21-31 or 46;
- and
- (b) a tryptamine derivative,
- wherein the engineered microbial cell converts a substituted tryptamine to the tryptamine derivative, wherein the tryptamine derivative is:
12 more elements
Dependencies are inferred from the claim text.