Haploidization in sorghum
AssigneeKWS SAAT SE & Co. KGaA
InventorsMonika Kloiber-Maitz, Silke Wieckhorst, Christof Bolduan, Milena Ouzunova
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Abstract
Sorghum plants are provided which are capable of inducing haploidy by modifications in the genome related to a pollen-specific expressed patatin phospholipid producing haploid offspring and can be produced for hybrid breeding in short time by chromosome doubling inbred lines, that is, homozygous father and mother lines. In addition, methods are provided for producing transgenic and non-transgenic plant haploid inducers and improving the induction performance of plants.
Claims13
A sorghum plant which is capable of inducing haploidy, wherein the plant has one or more modifications relating to an endogenous gene encoding a patatin phospholipase,
- wherein the patatin phospholipase is encoded by the nucleotide sequence according to SEQ ID NO:
- 1 or 2 or by a nucleotide sequence which is at least 95% identical to SEQ ID NO:
- 1 or 2, or which comprises the amino acid sequence shown in SEQ ID NO:
- 3 or a homologous amino acid sequence,
- wherein the one or more modifications are one or more mutations in the endogenous gene encoding the patatin phospholipase, and
- wherein the one or more mutations result in an amino acid exchange at least at amino acid position 291 according to SEQ ID NO:
- 3.
A nucleic acid molecule which encodes a mutated patatin phospholipase encoded by the nucleotide sequence according to SEQ ID NO:
- 1 or 2 or by a nucleotide sequence which is at least 95% identical to SEQ ID NO:
- 1 or 2, or which comprises the amino acid sequence shown in SEQ ID NO:
- 3 or a homologous amino acid sequence,
- wherein the one or more modifications are one or more mutations in the endogenous gene encoding the patatin phospholipase,
- wherein the one or more mutations results in an amino acid exchange at the amino acid position 291 according to SEQ ID NO:
- 3, and
- wherein the presence of the nucleic acid molecule in a plant in the absence of a wild-type patatin phospholipase results in the plant being able to induce haploidy.
A method for obtaining a plant capable of inducing haploidy or having an increased induction rate with respect to the wild type, comprising the following steps:
- (a) (i) mutagenizing plant cells and then regenerating plants from the mutagenized plant cells or mutagenizing plants;
- (ii) identifying a plant from (i) having one or more mutations in an endogenous DNA sequence which result in serine (S) at position 291 of the amino acid sequence according to SEQ ID NO:
- 3 being replaced by leucine (L) and capable of inducing the obtaining of haploid offspring at an increased rate compared to a non-mutagenized plant;
- or
- (b) (i) introducing the nucleic acid molecule having one or more mutations which result in serine (S) at position 291 of the amino acid sequence according to SEQ ID NO:
- 3 being replaced by leucine (L), in a plant cell, and
- (ii) regenerating a plant from the plant cell (i).
Dependencies are inferred from the claim text.