Targeted RNA editing by leveraging endogenous ADAR using engineered RNAs
AssigneePeking University
InventorsWensheng Wei, Zongyi Yi, Liang Qu, Feng Tian, Chunhui Wang, Shiyou Zhu, Zhuo Zhou
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Abstract
Provided are methods for editing RNA by introducing a deaminase-recruiting RNA in a host cell for deamination of an adenosine in a target RNA, deaminase-recruiting RNAs used in the RNA editing methods, compositions and kits comprising the same.
Claims25
A method for editing a target RNA comprising a target adenosine in a host cell, comprising introducing a construct comprising a nucleic acid encoding the dRNA into the host cell, wherein:
- (1) the dRNA comprises a targeting RNA sequence that is at least partially complementary to the target RNA,
- (2) the dRNA is capable of recruiting an adenosine deaminase acting on RNA (ADAR), and
- (3) the dRNA forms a circular RNA within the host cell, wherein the circular RNA recruits an ADAR to deaminate a target adenosine in the target RNA;
- wherein the dRNA further comprises:
- (i) a 3′ ligation sequence and a 5′ ligation sequence;
- or (ii) a 3′ twister ribozyme sequence linked to the 3′ end of the nucleic acid encoding the dRNA and a 5′ twister ribozyme sequence linked to the 5′ end of the nucleic acid encoding the dRNA.
A method for editing a target RNA comprising a target adenosine in a host cell, comprising introducing a deaminase-recruiting RNA (dRNA) into the host cell, wherein:
- (1) the dRNA comprises a targeting RNA sequence that is at least partially complementary to the target RNA,
- (2) the dRNA is capable of recruiting an adenosine deaminase acting on RNA (ADAR), and (3) the dRNA is a circular RNA, wherein the circular RNA recruits an ADAR to deaminate a target adenosine in the target RNA.
Dependencies are inferred from the claim text.