High intensity labeled reactant compositions and methods for sequencing
AssigneeQuantum-Si Incorporated
InventorsJonathan M. Rothberg, Jeremy Lackey, Brian Reed, Xinghua Shi, Haidong Huang, David Dodd
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Abstract
Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleic acid connected to a nucleotide and two or more luminescent labels. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided.
Claims18
A labeled molecule comprising a reactant connected to two or more luminescent labels via a nucleic acid linker, wherein each luminescent label is at least 5 angstroms separated from any other luminescent label, wherein
- (i) at least one luminescent label is integrated within the nucleic acid linker, and wherein the at least one luminescent label that is integrated within the nucleic acid linker is a cyanine-based fluorophore;
- and/or
- (ii) wherein at least one luminescent label is attached to the nucleic acid linker via a glycolamine spacer molecule, and wherein the labeled nucleotide comprises the structure:
- where is the at least one luminescent label that is attached to the nucleic acid linker via the glycolamine spacer molecule.
A labeled nucleotide comprising a nucleoside polyphosphate connected to two or more luminescent labels via a nucleic acid linker, wherein the nucleic acid linker is connected to the terminal phosphate of the nucleoside polyphosphate, wherein each luminescent label is at least 5 angstroms separated from any other luminescent label, wherein
- (i) at least one luminescent label is integrated into the nucleic acid linker, and wherein the at least one luminescent label that is integrated within the nucleic acid linker is a cyanine-based fluorophore;
- and/or
- (ii) wherein at least one luminescent label is attached to the nucleic acid linker via a glycolamine spacer molecule, and wherein the labeled nucleotide comprises the structure:
- where is the at least one luminescent label that is attached to the nucleic acid linker via the glycolamine spacer molecule.
Dependencies are inferred from the claim text.