Activated formylglycine-generating enzymes and methods of producing and using the same
AssigneeR.P. Scherer Technologies, LLC
InventorsDavid Rabuka, Gregory W. deHart, Patrick Holder, Jeanne Baker
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Abstract
The present disclosure provides activated formylglycine-generating enzymes (FGE), methods of producing activated FGE, and their use in methods of producing a protein comprising a formylglycine (FGly) residue. The methods of producing activated FGE, as well as methods of use of activated FGE in producing FGly-containing proteins, include both cell-based and cell-free methods. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure are also provided.
Claims10
An activated formylglycine-generating enzyme (FGE) produced by a method comprising treating an FGE with Cu2+ to produce an activated FGE.
A cell-free composition comprising:
- an activated formylglycine-generating enzyme (FGE);
- and
- a buffer.
A kit comprising:
- an activated formylglycine-generating enzyme (FGE);
- and
- instructions for using the activated FGE to convert a cysteine residue or a serine residue present in an FGE recognition site of a protein to a formylglycine residue.
A kit comprising:
- a nucleic acid that encodes a formylglycine-generating enzyme (FGE);
- and
- Cu2+ or a source of Cu2+.
Dependencies are inferred from the claim text.