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US 11,655,453 B2Granted
Expansion of γδ T cells, compositions, and methods of use thereof
AssigneesKing's College London, GammaDelta Therapeutics Ltd
InventorsRichard Beatson, Adrian Hayday, Oliver Nussbaumer, Richard Woolf, Maria Luisa Iannitto, Natalie Mount
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Abstract
The present invention provides methods of expanding γδ T cells from a non-haematopoietic tissue source. Further provided are compositions of expanded γδ T cells and methods of using the expanded γδ T cells (e.g., apart of an adoptive T cell therapy).
Claims17
A method of expanding γδ T cells, the method comprising the steps of:
- (i) providing a population of γδ T cells obtained from a non-haematopoietic tissue;
- and
- (ii) culturing the γδ T cells in the presence of:
- (a) IL-2 or IL-9;
- (b) IL-15;
- and
- (c) IL-21
- for at least 5 days in amounts effective to produce an expanded population of γδ T cells,
- wherein the method further comprises, after step (i), separating the γδ T cells from non-haematopoietic cells to produce a separated population of γδ T cells, and step (ii) comprises culturing the γδ T cells in the absence of substantial stromal cell contact;
- and
- wherein step (ii) comprises culturing the γδ T cells in the absence of exogenous TCR pathway agonists.
8 more elements
Dependencies are inferred from the claim text.