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US 11,649,493 B1Granted
Methods and systems for nucleic acid sequencing
AssigneeUltima Genomics, Inc.
InventorsMark Pratt, Florian Oberstrass, Linda Lee, Steven Menchen
Life of this patent
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Abstract
The present disclosure provides methods and systems for processing a nucleotide mixture. A nucleotide mixture can be purified. A nucleotide mixture can be processed for use in nucleic acid synthesis. A nucleotide mixture can be processed for use in nucleic acid sequencing.
Claims19
A method for processing a plurality of nucleic acid molecules, comprising:
- (a) directing a plurality of nucleotides or nucleotide analogs to a first reaction space comprising a support having a first plurality of nucleic acid molecules immobilized thereto;
- (b) incorporating a subset of nucleotides or nucleotide analogs from said plurality of nucleotides or nucleotide analogs into said first plurality of nucleic acid molecules, thereby providing a remainder of said plurality of nucleotides or nucleotide analogs, wherein (b) is performed in absence of detecting said subset of nucleotides incorporated into said first plurality of nucleic acid molecules;
- (c) bringing said remainder of said plurality of nucleotides or nucleotide analogs in contact with a second plurality of nucleic acid molecules in a second reaction space;
- and
- (d) incorporating at least a subset of said remainder of said plurality of nucleotides or nucleotide analogs into said second plurality of nucleic acid molecules.
2 more elements
Dependencies are inferred from the claim text.